SRF presents Repromatics series

Welcome to SRF presents Repromatics! Where we help biologists make peace with computational biology (yes, even the terrified ones). We’re kicking things off with a crash course in Whole Exome Sequencing analysis, no PhD in computer science required! In this 5-part monthly seminar series, we’ll show you how to use Galaxy, a wonderfully nerdy (and free!) web-based tool that makes data wrangling feel almost magical.

Episode One

In this episode, we’ll:

• Break down the basics of DNA sequencing Introduce the real-world clinical problem we’ll be tackling together
• Throw you headfirst into the Galaxy interface
• New episodes drop monthly, so hit that subscribe button and bring snacks.

Raw Data that members will need to follow along with the tutorial is below: https://drive.google.com/drive/folders/1QubGcvoB3Gi5SQG32s642HiRMwp3u0yh?usp=drive_link

Please note: When you click on the link, it will show 0 files but if you click download it will work.

Episode Two:

Welcome to Part 2 of the exome sequencing analysis tutorial series. In this session we focus on quality control, the step that determines whether sequencing data is reliable enough for accurate variant detection. Using FastQC in the Galaxy platform, we explain how to interpret the main QC plots and recognise signs of bias, contamination, or technical issues, and when data should be cleaned or even resequenced.